Coding

Part:BBa_K343100:Design

Designed by: Louise Linnebjerg Bohn Christoffersen, Pernille Marie Madsen, Sheila Maibom-Thomsen   Group: iGEM10_SDU-Denmark   (2010-10-17)

Flagella master regulating operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 821
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 821
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 821
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 821
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has an internal Pst1 restriction site in the flhC part of the operon. To remove this we also designed a Pst1-free brick: part: BBa_343000 and a composite part BBa_K343004, where the restiction site has been removed by introducing a silent mutation.

Source

It is a natural part of the E.coli genome

References